Celebrating 20 years at KML

Hot off the printing presses! New KML 20th Anniversary t-shirts! Designed by KML alum, Peter Bouwma (Bouwma Communication Design), with the KML 20th Anniversary logo on the front pocket area and full-color artwork depicting marine creatures of the Keys, bayside and oceanside, on the back (can you find KML on the map?). These beautiful 100% heavy-weight (6.2 oz) cotton tees are available in sizes Small through XX-Large. Short-sleeved style available in white or ash for $15.00 (plus $1.13 tax). Long-sleeved tees available in ash only for $20.00 (plus $1.50 tax). Order yours online through the Wildlife Foundation of Florida http://www.wildlifefoundationofflorida.com/?q=catalog/1

If you are visiting KML, order your shirts through the Foundation, present your receipt, and pick up your shirt in the office. KML ball caps are also still available for $15.00, plus tax.

As always, your generous donations are used for upgrades and improvements around the Lab and are tremendously appreciated.

Welcome Trevor, new KML staff scientist

Trevor Luna joined the KML team as a Biological Scientist for FWC/FWRI in December of 2011. He graduated with a Bachelors of Arts in Biology from Rollins College in Winter Park, FL before moving to Zambia, Africa to serve in the U.S. Peace Corps. There he worked with the Zambian Department of Livestock and Fisheries as an Aquaculture Extension Agent promoting rural Tilapia culture. Trevor is an avid boater, diver, fisherman and hunter. The KML team is very excited to have Trevor on board. Welcome Trevor!

Spiny lobster denning behavior

What factors contribute to the denning behavior of Florida Bay's juvenile spiny lobster (Panularis argus) population? And how might shelter loss events, such as sponge die-offs, influence denning behavior and dispersal? What is the most effective way to conserve this important marine resource?
Katherine (Kat) Heldt, PhD candidate from Clemson University, has spent several months at KML, observing "social status" among lobsters and testing what mechanisms by which they choose shelters. Kat hopes to determine whether dominance status or familiarity can influence denning behavior and dispersal.

First, pairs of lobsters are housed in 15-gal replicate aquaria, plumbed to KML's seawater system. They are offered artificial shelter and observed at night for aggressive behavior under dim red lighting.

Next, the lobsters are transferred to KML's mesocosm ("The Shallows", KML's 122,000-gal flow-thru seawater pond) along with unfamiliar individuals (pairs housed in separate aquaria) equipped with artificial shelters. After several days of observing denning behavior and dispersal in the mesocosm, some of the shelter blocks are removed to mimic sudden habitat loss.

Finally, the tagged lobsters are released onto field sites (16 pre-selected 25m x 25m near-shore bay-side sites) for further observation.

Kat and her lab assistant, Frank, self-captain KML's 18' Parker, the R/V NariNari, to reach their sites.

FAU students take a fish-eye view of marine habitats

No, this is not synchronized swim class. Dr. Stephen Kajiura, professor at Florida Atlantic University, brought 17 graduate students down to KML for their Sensory Biology and Behavior of Fish field trip in October. They spent 3 days exploring the various marine habitats, observing and discussing fish behavior.
Moving off the reef into deep water, students paired up to see how well they could navigate (ie swim a straight line) without visual references, such as being able to see the bottom. Students all agreed that they would make very poor fish. In another exercise, they were challenged to detect sounds of varying frequencies, while under water at varying depths and distances from the sound source.
Dr. Kajiura and his grad students are frequent visitors to KML, studying elasmobranch sensory abilities.

BURR Team on hand for Coral Spawning Event in the Keys

Boulder Star Coral (Montastrea faveolata) setting gamete bundles prior to spawning (photo by P. Gillet)

Every August, several nights after the full moon, Boulder Corals along the Florida Keys reef tract synchronize their reproductive efforts in an amazing coral spawning event. Dr. Mary Alice Coffroth, professor at SUNY Buffalo, was on hand to capture the event for on-going projects exploring the many facets of coral-algal symbiosis.

An army of grad students, divers and snorkelers from Buffalo as well as volunteers from south Florida institutions, took to the water to arrange collection tents over promising colonies near Alligator Reef as dusk approached, then returned to the boat to wait. Teams of divers splashed again at 11:00pm to gather cupfuls of the coral spawn, handing them off to snorkelers who ferried them back to the boat. Weather was a bit rough but the team was fearless!

Returning to the dock at 2:00am, the army worked around the clock at KML's wet lab facility to carefully rear the developing coral larvae. Spawning was so successful at the Middle Keys site this year, that the BURR Team (Buffalo Undersea Reef Research) had plenty to share with fellow scientists in the Upper and Lower Keys.

The new coral recruits were allowed to settle on ceramic tiles in their special Kreisells at the wet lab and then placed back out on the reef to follow development. Dr. Coffroth's team will periodically return to KML to sample the new recruits on the tiles to asses the coral/algal symbiosis between near-shore and off-shore sites.

Check out the BURR blog for more on this project
: http://burrcoralspawn11.blogspot.com/

The world of the Upside-down Jellyfish

Upside-down jellyfish, Cassiopea xamachana

Rachel Mellas, Masters candidate at SUNY Buffalo with Dr. Mary Alice Coffroth, spent 2 months at KML. She is studying the fitness effects and symbiont type in the upside-down jellyfish, Cassiopea xamachana.

Upside-down jellyfish right side-up!

In Cassiopea, establishment of the symbiosis occurs in the scyphistomae (polyp) stage of development where multiple strains of Symbiodinium can be acquired. Upon infection, the scyphistomae produce ephyra (young medusa) through a process termed strobilation. Once they reach the adult medusa form, they typically harbor one specific type of symbiont (Symbiodinium A1).

Cassiopea scyphistomae (polyp) stage

To understand the potential fitness advantages to the host of harboring different symbiont types, Rachel has set up laboratory experiments which look at the how different symbionts affect the growth rate, survivorship, and timing of strobilation of scyphistomae, and if strobilation occurs with only certain symbionts (A1). (photos by R Mellas)